《植物生理学报》 2010, 46(6): 529-536

耐盐杂草稻3 个锌指蛋白基因家族的实时定量分

张丽丽, 孙健, 马殿荣, 陈温福*

沈阳农业大学水稻研究所, 农业部北方作物生理生态重点开放实验室, 辽宁省北方粳稻遗传育种重点实验室, 沈阳110866

通信作者：陈温福；E-mail: wfchen5512@yahoo.com.cn；Tel: 024-88487184

摘　要：

利用在300 余份来源于辽宁、吉林、黑龙江、内蒙古、江苏等地的杂草稻材料中筛选出耐高盐杂草稻材料WR03-12。 通过 RT-PCR 的方法得到盐胁迫下 WR03-12 与盐敏感栽培稻 ‘ 越光’ 幼苗的cDNA 第一链, 对3 个锌指蛋白基因家族的6 个 基因表达情况进行了荧光实时定量分析。结果表明, 2 个 C2C2 型锌指蛋白基因 SRZ1 与 SRZ2 受到高盐胁迫的负向诱导, WR03-12 受负向诱导程度要小于 ‘ 越光 ’; 2 个 TFIIIA 型锌指蛋白基因 ZFP18 与 ZFP245 受到盐胁迫的正向诱导, WR03-12 受诱导程度也小于 ‘ 越光’; 具有 A20 锌指结构的基因AACZ1 基因在越光中不受盐诱导, 而在WR03-12 中受短时间诱导后, 第 7 天已经恢复到胁迫前水平。具有 AN1 锌指结构的基因 AACZ2 在 ‘ 越光 ’ 与 WR03-12 中均不受盐胁迫诱导, 且表达水 平没有显著差别。杂草稻 WR03-12 与 ‘ 越光 ’ 对于盐胁迫的应答机制可能在转录调控方面存在差别。

关键词：杂草稻; 锌指蛋白; 转录调控; 实时定量PCR

收稿：2010-03-16   修定：2010-04-16

资助：国家自然科学基金项目(30671262)、教育部高等学校博士学科点专项科研基金项目(20060157003)和水稻生物学国家重点实验室开放课题

Real-Time Quantitative Analysis on Three Zinc Finger Protein Gene Family of Salt-Tolerant Weedy Rice (Oryza sativa f. spontanea)

ZHANG Li-Li, SUN Jian, MA Dian-Rong, CHEN Wen-Fu*

Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Northern Japonica Rice Breeding of Liaoning, Rice Institute, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: CHEN Wen-Fu; E-mail: wfchen5512@yahoo.com.cn; Tel: 024-88487184

Abstract:

Salt-torlerant weedy rice WR03-12 was screend from 300 accessions derived from Liaoning, Jilin, Heilongjiang, Inner Mongolia and Jiangsu Province. To investigete the salt-tolerant mechanism of WR03-12 in transcriptional regulation level, the first strand cDNA of salt-sensitive varietiy ‘Koshihikari’ and salt-tolerant weedy rice WR03-12 were obtained, and the expression of six gene in three zinc finger protein family of the two germplasm was studied by Real-time quantitative PCR. Results showed (i) the C₂C₂ pattern gene of SRZ1 and SRZ2 was induced negatively by high salt stress, and the expression level in WR03-12 was lower than ‘Koshihikari’; (ii) the TFIIIA pattern gene of ZFP18 and ZFP245 was induced positively by high salt stress, and the expression level in WR03-12 was lower than ‘Koshihikari’; (iii) the expression of A20 pattern gene AACZ1 was not induced in ‘Koshihikari’, while was induced in a short time (7 day) in WR03-12 under high salt stress; (iv) the expression of AN1 pattern gene AACZ2 was not induced by high salt stress in both ‘Koshihikari’ and WR03-12. Results suggested that the salt stress response mechanism between WR03-12 and ‘Koshihikari’ may be different in transcriptional regulation.

Key words: weedy rice; zinc finger protein; transcriptional regulation; real time PCR